Composite

Part:BBa_K5206008:Design

Designed by: Yaoyao Liang   Group: iGEM24_Nanjing-BioX   (2024-09-30)


PrT7-ctrpiB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 487
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 228
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This Part can synthesize D-allose using D-allulose as a substrate. After codon optimization, it can be co-transformed with the biosensor into Escherichia coli BL21(DE3), which can verify the practical application of the biosensor. pET-28a(+) is used to construct recombinant plasmids to overexpress this gene.


Source

None

References